pCDB278

80694 Synthetic Bacterial Expression

Plasmid Information

Purpose
for secretion of N-terminal OsmY, deca-histidine, SUMO tagged gHH_44
Depositor
David Baker
Article
Bhardwaj et al Nature. 2016 Sep 14. doi: 10.1038/nature
Insert
gHH_44
Tags
OsmY-10His-Smt3
Promoter
T7
Available Since
Nov. 3, 2016
Availability
Available

Sequence Information

Length
1,020 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
10xHis — 10xHis affinity tag 653..683 Fwd
DNA Sequence
CACCACCACCACCACCACCACCACCACCAC
Protein Sequence
HHHHHHHHHH