pMTB2-cytoBirA-2A-mCherry_Ras

80066 Danio rerio Bacterial Expression

Plasmid Information

Purpose
Beta-actin2 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
Depositor
Tatjana Sauka-Spengler
Article
Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
Insert
Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
Tags
3x HA
Promoter
Beta-actin2
Available Since
Oct. 11, 2016
Availability
Available

Sequence Information

Length
947 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 3
Label Gene Product Position Strand
HA — HA (human influenza hemagglutinin) epit… 87..114 Fwd
DNA Sequence
TATGACGTCCCAGATTACGCAAGTTTG
Protein Sequence
YDVPDYASL
HA — HA (human influenza hemagglutinin) epit… 120..147 Fwd
DNA Sequence
TATGATGTTCCTGATTATGCTAGCCTC
Protein Sequence
YDVPDYASL
HA — HA (human influenza hemagglutinin) epit… 153..180 Fwd
DNA Sequence
TACGATGTGCCCGACTATGCCTCACTT
Protein Sequence
YDVPDYASL