pMT-zic2a-NLS-BirA-2A-mCherry_Ras

80065 Danio rerio Bacterial Expression

Plasmid Information

Purpose
Zic2a promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
Depositor
Tatjana Sauka-Spengler
Article
Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
Insert
Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
Tags
3x HA
Promoter
Zic2a enhancer driving c-fos promoter
Available Since
Oct. 11, 2016
Availability
Available

Sequence Information

Length
883 bp
Topology
linear
Molecule Type
genomic DNA

Features

protein_bind 1
Label Gene Product Position Strand
attB2 mutant version of attB 224..249 Fwd
DNA Sequence
ACCACTTTGTACAAGAAAGCTGGGT