pMT-MCS-NLS-BirA-2A-mCherry_Ras

Plasmid Information

Purpose

MCS for cloning promoter to drive HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

Depositor

Tatjana Sauka-Spengler

Article

Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

Insert

Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein

Sequence Information

Length

907 bp

Topology

linear

Molecule Type

genomic DNA

View on AddGene.org

Features

CDS 4

Label	Gene	Product	Position	Strand
HA	—	HA (human influenza hemagglutinin) epit…	385..412	Fwd
DNA Sequence TATGACGTCCCAGATTACGCAAGTTTG Protein Sequence YDVPDYASL
HA	—	HA (human influenza hemagglutinin) epit…	418..445	Fwd
DNA Sequence TATGATGTTCCTGATTATGCTAGCCTC Protein Sequence YDVPDYASL
HA	—	HA (human influenza hemagglutinin) epit…	451..478	Fwd
DNA Sequence TACGATGTGCCCGACTATGCCTCACTT Protein Sequence YDVPDYASL
SV40 NLS	—	nuclear localization signal of SV40 (si…	493..514	Fwd
DNA Sequence CCAAAAAAGAAGAGAAAGGTA Protein Sequence PKKKRKV

promoter 1

Label	Gene	Product	Position	Strand
SP6 promoter	—	—	290..309	Fwd
DNA Sequence ATTTAGGTGACACTATAGA