pMT-MCS-cytoBirA-2A-mCherry_Ras

80058 Danio rerio Bacterial Expression

Plasmid Information

Purpose
MCS for cloning promoter to drive HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
Depositor
Tatjana Sauka-Spengler
Article
Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
Insert
Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
Tags
3x HA
Available Since
Oct. 11, 2016
Availability
Available

Sequence Information

Length
957 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 3
Label Gene Product Position Strand
HA — HA (human influenza hemagglutinin) epit… 383..410 Fwd
DNA Sequence
TATGACGTCCCAGATTACGCAAGTTTG
Protein Sequence
YDVPDYASL
HA — HA (human influenza hemagglutinin) epit… 416..443 Fwd
DNA Sequence
TATGATGTTCCTGATTATGCTAGCCTC
Protein Sequence
YDVPDYASL
HA — HA (human influenza hemagglutinin) epit… 449..476 Fwd
DNA Sequence
TACGATGTGCCCGACTATGCCTCACTT
Protein Sequence
YDVPDYASL
promoter 1
Label Gene Product Position Strand
SP6 promoter — — 288..307 Fwd
DNA Sequence
ATTTAGGTGACACTATAGA