pGEM-NLS-BirA-2A-mCherry-SV40pA-FKF

79889 Danio rerio Bacterial Expression

Plasmid Information

Purpose
Donor cassette containing HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene
Depositor
Tatjana Sauka-Spengler
Article
Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
Insert
HA-tagged NLS-BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
Available Since
Oct. 11, 2016
Availability
Available

Sequence Information

Length
943 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 4
Label Gene Product Position Strand
HA HA (human influenza hemagglutinin) epit… 30..57 Fwd
DNA Sequence
TATGACGTCCCAGATTACGCAAGTTTG
Protein Sequence
YDVPDYASL
HA HA (human influenza hemagglutinin) epit… 63..90 Fwd
DNA Sequence
TATGATGTTCCTGATTATGCTAGCCTC
Protein Sequence
YDVPDYASL
HA HA (human influenza hemagglutinin) epit… 96..123 Fwd
DNA Sequence
TACGATGTGCCCGACTATGCCTCACTT
Protein Sequence
YDVPDYASL
SV40 NLS nuclear localization signal of SV40 (si… 138..159 Fwd
DNA Sequence
CCAAAAAAGAAGAGAAAAGTA
Protein Sequence
PKKKRKV