pMT-ubb-Avi-Cerulean-Rpl10a

79882 Danio rerio Bacterial Expression

Plasmid Information

Purpose
Ubiquitin promoter driving Avi-tagged protein containing Cerulean protein fused to zebrafish Rpl10a ribosomal unit (Tryon et al. 2012); flanked by Tol2 sequences
Depositor
Tatjana Sauka-Spengler
Article
Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
Insert
Cerulean protein fused to zebrafish Rpl10a ribosomal unit (rpl10a Zebrafish)
Tags
Avi
Promoter
Ubiquitin
Available Since
Oct. 11, 2016
Availability
Available

Sequence Information

Length
920 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

RBS 1
Label Gene Product Position Strand
Shine-Dalgarno sequence — — 650..659 Fwd
DNA Sequence
ACCTCCTTA
promoter 1
Label Gene Product Position Strand
T7 promoter — — 3..22 Fwd
DNA Sequence
TAATACGACTCACTATAGG