pMTB2-Avi-Cerulean-Rpl10a

79880 Danio rerio Bacterial Expression

Plasmid Information

Purpose
Beta-actin2 promoter driving Avi-tagged protein containing Cerulean protein fused to zebrafish Rpl10a ribosomal unit (Tryon et al. 2012); flanked by Tol2 sequences
Depositor
Tatjana Sauka-Spengler
Article
Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
Insert
Cerulean protein fused to zebrafish Rpl10a ribosomal unit (rpl10a Zebrafish)
Tags
Avi
Promoter
Beta-actin2
Available Since
Oct. 11, 2016
Availability
Available

Sequence Information

Length
923 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 3
Label Gene Product Position Strand
AviTag(TM) peptide tag that allows for enzymatic b… 23..68 Fwd
DNA Sequence
GGCCTGAATGACATCTTTGAGGCCCAGAAGATCGAGTGGCATGAG
Protein Sequence
GLNDIFEAQKIEWHE
TEV site tobacco etch virus (TEV) protease recog… 74..95 Fwd
DNA Sequence
GAGAACCTGTACTTCCAGGGA
Protein Sequence
ENLYFQG
mCerulean enhanced monomeric variant of CFP (Rizz… 98..815 Fwd
DNA Sequence
ATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTGGGGCGTGCAGTGCTTCGCCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACGCCATCAGCGACAACGTCTATATCACCGCCGACAAGCAGAAGAACGGCATCAAGGCCAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCAAGCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAG
Protein Sequence
MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK
RBS 1
Label Gene Product Position Strand
Shine-Dalgarno sequence 898..907 Fwd
DNA Sequence
TAAGGAGGT