HASP_HUMAN_D0

79730 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
This plasmid encodes the kinase domain of HASP. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
Depositor
John Chodera, Nicholas Levinson, Markus Seeliger
Article
Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/
Insert
HASP
Tags
His10-TEV
Promoter
T7
Available Since
Nov. 21, 2016
Availability
Available

Sequence Information

Length
871 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 4
Label Gene Product Position Strand
10xHis 10xHis affinity tag 97..127 Fwd
DNA Sequence
CACCATCACCATCACCATCACCATCACCAT
Protein Sequence
HHHHHHHHHH
10xHis 10xHis affinity tag 100..130 Fwd
DNA Sequence
CATCACCATCACCATCACCATCACCATCAC
Protein Sequence
HHHHHHHHHH
10xHis 10xHis affinity tag 103..133 Fwd
DNA Sequence
CACCATCACCATCACCATCACCATCACCAT
Protein Sequence
HHHHHHHHHH
TEV site tobacco etch virus (TEV) protease recog… 133..154 Fwd
DNA Sequence
GAAAACCTGTACTTCCAATCC
Protein Sequence
ENLYFQS