DMPK_HUMAN_D0

79717 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
This plasmid encodes the kinase domain of DMPK. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
Depositor
John Chodera, Nicholas Levinson, Markus Seeliger
Article
Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/
Insert
DMPK (DMPK Human)
Tags
His10-TEV
Promoter
T7
Available Since
Nov. 21, 2016
Availability
Available

Sequence Information

Length
948 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
10xHis — 10xHis affinity tag 100..130 Fwd
DNA Sequence
CACCATCACCATCACCATCACCATCACCAT
Protein Sequence
HHHHHHHHHH
TEV site — tobacco etch virus (TEV) protease recog… 130..151 Fwd
DNA Sequence
GAAAACCTGTACTTCCAATCC
Protein Sequence
ENLYFQS
RBS 1
Label Gene Product Position Strand
Shine-Dalgarno sequence — — 309..318 Fwd
DNA Sequence
TAAGGAGGT