CSK_HUMAN_D0 — PlasmidDB

Plasmid Information

Purpose

This plasmid encodes the kinase domain of CSK. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

CSK (CSK Human)

Sequence Information

Length

252 bp

Topology

linear

Molecule Type

genomic DNA

View on AddGene.org

Features

CDS 2

Label	Gene	Product	Position	Strand
10xHis	—	10xHis affinity tag	102..132	Fwd
DNA Sequence CACCATCACCATCACCATCACCATCACCAT Protein Sequence HHHHHHHHHH
TEV site	—	tobacco etch virus (TEV) protease recog…	132..153	Fwd
DNA Sequence GAAAACCTGTACTTCCAATCC Protein Sequence ENLYFQS