pRS315e_pGal-dCas9-SH3-pGal-PmCDA1-SHL

79614 Streptococcus pyogenes Yeast Expression

Plasmid Information

Purpose
Expresses dCas9-SH3 and PmCDA1-SHL in yeast cells
Depositor
Akihiko Kondo
Article
Nishida et al Science. 2016 Aug 4. pii: aaf8729.
Tags
SH3 domain and SHL
Mutation
D10A and H840A for nuclease deficient Cas9
Promoter
pGal1 and pGal10
Available Since
July 27, 2016
Availability
Available

Sequence Information

Length
780 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 2
Label Gene Product Position Strand
3xFLAG three tandem FLAG(R) epitope tags, foll… 55..121 Rev
DNA Sequence
GACTATAAGGACCACGACGGAGACTACAAGGATCATGATATTGATTACAAAGACGATGACGATAAG
Protein Sequence
DYKDHDGDYKDHDIDYKDDDDK
SV40 NLS nuclear localization signal of SV40 (si… 328..349 Rev
DNA Sequence
CCCAAGAAGAAGAGGAAGGTG
Protein Sequence
PKKKRKV