5H9M (SIAH2)

79521 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Bacterial expression for structure determination; may not be full ORF
Depositor
Cheryl Arrowsmith
Insert
SIAH2 (SIAH2 Human)
Tags
8xHis-Thrombin-SUMO-TEV
Promoter
T7-lacO
Available Since
June 15, 2016
Availability
Available

Sequence Information

Length
838 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 4
Label Gene Product Position Strand
8xHis 8xHis affinity tag 30..54 Fwd
DNA Sequence
CATCACCATCATCATCATCATCAC
Protein Sequence
HHHHHHHH
thrombin site thrombin recognition and cleavage site 63..81 Fwd
DNA Sequence
CTGGTTCCGCGTGGTAGT
Protein Sequence
LVPRGS
SUMO S. cerevisiae SMT3 (truncated) cleavable ubiquitin-like protein tag 81..375 Fwd
DNA Sequence
ATGTCGGACTCAGAAGTCAATCAAGAAGCTAAGCCAGAGGTCAAGCCAGAAGTCAAGCCTGAGACTCACATCAATTTAAAGGTGTCCGATGGATCTTCAGAGATCTTCTTCAAGATCAAAAAGACCACTCCTTTAAGAAGGCTGATGGAAGCGTTCGCTAAAAGACAGGGTAAGGAAATGGACTCCTTAAGATTCTTGTACGACGGTATTAGAATCCAAGCTGATCAGACCCCTGAAGATTTGGACATGGAGGATAACGATATTATTGAGGCTCACAGAGAACAGATTGGTGGA
Protein Sequence
MSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLRRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIGG
TEV site tobacco etch virus (TEV) protease recog… 378..399 Fwd
DNA Sequence
GAAAACTTGTATTTCCAGGGC
Protein Sequence
ENLYFQG