pAB045a

79224 Synthetic Bacterial Expression

Plasmid Information

Purpose
Directed Evolution of Protein-Protein Interactions
Depositor
David Liu
Article
Badran et al Nature. 2016 May 5;533(7601):58-63. doi:
Insert
PlacZ rpoZ-HA4; PlacIq lacI
Promoter
PlacZ; PlacIq
Available Since
Oct. 21, 2016
Availability
Available

Sequence Information

Length
800 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 1
Label Gene Product Position Strand
RNAP omega E. coli rpoZ DNA-directed RNA polymerase subunit ome… 511..784 Fwd
DNA Sequence
ATGGCACGCGTAACTGTTCAGGACGCTGTAGAGAAAATTGGTAACCGTTTTGACCTGGTACTGGTCGCCGCGCGTCGCGCTCGTCAGATGCAGGTAGGCGGAAAGGATCCGCTGGTACCGGAAGAAAACGATAAAACCACTGTAATCGCGCTGCGCGAAATCGAAGAAGGTCTGATCAACAACCAGATCCTCGACGTTCGCGAACGCCAGGAACAGCAAGAGCAGGAAGCCGCTGAATTACAAGCCGTTACCGCTATTGCTGAAGGTCGTCGT
Protein Sequence
MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEENDKTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR
promoter 1
Label Gene Product Position Strand
lac promoter 418..449 Fwd
DNA Sequence
TTTACACTTTATGCTTCCGGCTCGTATGTTG
protein_bind 2
Label Gene Product Position Strand
CAP binding site 382..404 Fwd
DNA Sequence
TAATGTGAGTTAGCTCACTCAT
lac operator 456..473 Fwd
DNA Sequence
TTGTGAGCGGATAACAA