pAB031a

79221 Synthetic Bacterial Expression

Plasmid Information

Purpose
Directed Evolution of Protein-Protein Interactions
Depositor
David Liu
Article
Badran et al Nature. 2016 May 5;533(7601):58-63. doi:
Insert
PlacZ HA4-Zif268; PlacIq lacI
Promoter
PlacZ; PlacIq
Available Since
March 30, 2017
Availability
Available

Sequence Information

Length
786 bp
Topology
linear
Molecule Type
genomic DNA

Features

promoter 1
Label Gene Product Position Strand
lac promoter 396..427 Fwd
DNA Sequence
TTTACACTTTATGCTTCCGGCTCGTATGTTG
protein_bind 2
Label Gene Product Position Strand
CAP binding site 360..382 Fwd
DNA Sequence
TAATGTGAGTTAGCTCACTCAT
lac operator 434..451 Fwd
DNA Sequence
TTGTGAGCGGATAACAA