pET3a-OSF-CypA

79039 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Bacterial vector for cloning and propagation in DH5alpha
Depositor
Wesley Sundquist
Article
Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1
Insert
OneSTrEP-FLAG- Cyclophilin A (PPIA Human)
Tags
OneSTrEP-FLAG
Promoter
T7
Available Since
Jan. 30, 2017
Availability
Available

Sequence Information

Length
516 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 3
Label Gene Product Position Strand
Strep-Tag II peptide that binds Strep-Tactin(R), an … 36..60 Fwd
DNA Sequence
TGGAGCCACCCGCAGTTCGAAAAA
Protein Sequence
WSHPQFEK
FLAG FLAG(R) epitope tag, followed by an ent… 66..90 Fwd
DNA Sequence
GATTACAAGGATGACGATGACAAG
Protein Sequence
DYKDDDDK
HRV 3C site recognition and cleavage site for human… 105..129 Fwd
DNA Sequence
CTGGAAGTCCTGTTCCAGGGCCCC
Protein Sequence
LEVLFQGP