pGEX6P1-Ubl

78712 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Expresses Sacsin Ubl- domain (2-85) in fusion with GST (bacterial)
Depositor
Kalle Gehring
Article
Menade et al J Biol Chem. 2018 Aug 17;293(33):12832-1
Insert
Human Sacsin 2-85
Tags
GST-Tag
Available Since
July 19, 2016
Availability
Available

Sequence Information

Length
921 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 1
Label Gene Product Position Strand
HRV 3C site recognition and cleavage site for human… 8..32 Fwd
DNA Sequence
CTGGAAGTTCTGTTCCAGGGGCCC
Protein Sequence
LEVLFQGP
promoter 1
Label Gene Product Position Strand
AmpR promoter bla 614..719 Fwd
DNA Sequence
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT