pSRK2721 — PlasmidDB

Plasmid Information

Purpose

Gateway destination vector for production of MBP-3C site-His6-TEV site-passenger protein fusions

Depositor

David Waugh

Article

Raran-Kurussi et al Anal Biochem. 2016 Apr 19;504:30-37. doi

Insert

Gateway cloning cassette

Available Since

Sept. 27, 2016

Availability

Available

Length

716 bp

Topology

linear

Molecule Type

genomic DNA

protein_bind 1

Label	Gene	Product	Position	Strand
attR2	mutant version of attR	—	86..211	Rev
DNA Sequence ACCACTTTGTACAAGAAAGCTGAACGAGAAACGTAAAATGATATAAATATCAATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAAAACACAACATATCCAGTCACTATG

terminator 1

Label	Gene	Product	Position	Strand
rrnB T1 terminator	Escherichia coli rrnB	—	587..674	Fwd
DNA Sequence CAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAAT