His10-CD3z-SNAPf-ybbR (XSB507)

78508 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Construct for expressing CD3z-SNAPf-ybbR in E. Coli
Depositor
Ron Vale
Article
Su et al Science. 2016 Apr 29;352(6285):595-9. do
Insert
CD3z (Cytoplasmic domain) (CD247 Human)
Tags
His10 and SNAPf-ybbR
Available Since
June 16, 2016
Availability
Available

Sequence Information

Length
1,053 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
10xHis — 10xHis affinity tag 486..516 Fwd
DNA Sequence
CATCATCATCATCATCACCATCACCATCAC
Protein Sequence
HHHHHHHHHH
promoter 2
Label Gene Product Position Strand
lacI promoter lacI — 1..79 Rev
DNA Sequence
GACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAAT
T7 promoter — — 387..406 Fwd
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 1
Label Gene Product Position Strand
lac operator — — 406..431 Fwd
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC