HisMBP CFlm25

78458 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Inducible expression in E. coli
Depositor
Sylvie Doublie
Article
Coseno et al Nucleic Acids Res. 2008 Jun;36(10):3474-
Insert
Human Cleavage Factor lm 25 kDa subunit (NUDT21 Human)
Tags
His and MBP
Promoter
tac
Available Since
July 22, 2016
Availability
Available

Sequence Information

Length
1,011 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
TEV site — tobacco etch virus (TEV) protease recog… 13..34 Fwd
DNA Sequence
GAGAACCTGTACTTCCAGGGT
Protein Sequence
ENLYFQG
protein_bind 1
Label Gene Product Position Strand
attB2 mutant version of attB — 720..745 Rev
DNA Sequence
ACCACTTTGTACAAGAAAGCTGGGT