pNX3a-FL3

78358 Schizosaccharomyces pombe Yeast Expression

Plasmid Information

Purpose
fission yeast epitope tagging
Depositor
Kazunori Tomita
Article
Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.
Available Since
Nov. 17, 2016
Availability
Available

Sequence Information

Length
887 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
FLAG — FLAG(R) epitope tag, followed by an ent… 42..66 Fwd
DNA Sequence
GACTACAAGGACGACGATGACAAG
Protein Sequence
DYKDDDDK
2xFLAG — two tandem FLAG(R) epitope tags 69..117 Fwd
DNA Sequence
GATTACAAAGATGACGACGATAAGGACTACAAGGACGACGATGACAAG
Protein Sequence
DYKDDDDKDYKDDDDK
promoter 1
Label Gene Product Position Strand
TEF promoter — — 392..736 Fwd
DNA Sequence
GACATGGAGGCCCAGAATACCCTCCTTGACAGTCTTGACGTGCGCAGCTCAGGGGCATGATGTGACTGTCGCCCGTACATTTAGCCCATACATCCCCATGTATAATCATTTGCATCCATACATTTTGATGGCCGCACGGCGCGAAGCAAAAATTACGGCTCCTCGCTGCAGACCTGCGAGCAGGGAAACGCTCCCCTCACAGACGCGTTGAATTGTCCCCACGCCGCGCCCCTGTAGAGAAATATAAAAGGTTAGGATTTGCCACTGAGGTTCTTCTTTCATATACTTCCTTTTAAAATCTTGCTAGGATACAGTTCTCACATCACATCCGAACATAAACAACC
terminator 1
Label Gene Product Position Strand
ADH1 terminator S. cerevisiae ADH1 — 146..334 Fwd
DNA Sequence
GCGAATTTCTTATGATTTATGATTTTTATTATTAAATAAGTTATAAAAAAAATAAGTGTATACAAATTTTAAAGTGACTCTTAGGTTTTAAAACGAAAATTCTTATTCTTGAGTAACTCTTTCCTGTAGGTCAGGTTGCTTTCTCAGGTATAGTATGAGGTCGCTCTTATTGACCACACCTCTACCGG