pNX3a-PK3

78346 Schizosaccharomyces pombe Yeast Expression

Plasmid Information

Purpose
fission yeast epitope tagging
Depositor
Kazunori Tomita
Article
Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.
Available Since
Nov. 17, 2016
Availability
Available

Sequence Information

Length
913 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 3
Label Gene Product Position Strand
V5 tag epitope tag from simian virus 5 32..74 Fwd
DNA Sequence
GGTAAGCCTATTCCTAACCCTCTTTTGGGTCTCGATTCTACA
Protein Sequence
GKPIPNPLLGLDST
V5 tag epitope tag from simian virus 5 77..119 Fwd
DNA Sequence
GGAAAACCAATCCCCAACCCACTCCTCGGCCTTGACTCAACT
Protein Sequence
GKPIPNPLLGLDST
V5 tag epitope tag from simian virus 5 119..161 Fwd
DNA Sequence
GGCAAACCCATACCAAATCCTTTACTTGGATTAGATAGTACC
Protein Sequence
GKPIPNPLLGLDST
promoter 1
Label Gene Product Position Strand
TEF promoter 436..780 Fwd
DNA Sequence
GACATGGAGGCCCAGAATACCCTCCTTGACAGTCTTGACGTGCGCAGCTCAGGGGCATGATGTGACTGTCGCCCGTACATTTAGCCCATACATCCCCATGTATAATCATTTGCATCCATACATTTTGATGGCCGCACGGCGCGAAGCAAAAATTACGGCTCCTCGCTGCAGACCTGCGAGCAGGGAAACGCTCCCCTCACAGACGCGTTGAATTGTCCCCACGCCGCGCCCCTGTAGAGAAATATAAAAGGTTAGGATTTGCCACTGAGGTTCTTCTTTCATATACTTCCTTTTAAAATCTTGCTAGGATACAGTTCTCACATCACATCCGAACATAAACAACC
terminator 1
Label Gene Product Position Strand
ADH1 terminator S. cerevisiae ADH1 190..378 Fwd
DNA Sequence
GCGAATTTCTTATGATTTATGATTTTTATTATTAAATAAGTTATAAAAAAAATAAGTGTATACAAATTTTAAAGTGACTCTTAGGTTTTAAAACGAAAATTCTTATTCTTGAGTAACTCTTTCCTGTAGGTCAGGTTGCTTTCTCAGGTATAGTATGAGGTCGCTCTTATTGACCACACCTCTACCGG