pTol2p2A--3mnx1:loxP-mTagBFP2-stop-loxP-mCherry-NS-MCS

Plasmid Information

Purpose

Can be used to subclone in tagged gene of interest into inducible motor neuron expression vector.

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Promoter

-3mnx1

Available Since

Oct. 6, 2016

Availability

Available

Length

1,052 bp

Topology

linear

Molecule Type

genomic DNA

polyA_signal 1

Label	Gene	Product	Position	Strand
SV40 poly(A) signal	—	—	266..401	Fwd
DNA Sequence GATCCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTT

protein_bind 2

Label	Gene	Product	Position	Strand
loxP	—	—	454..488	Rev
DNA Sequence ATAACTTCGTATAATGTATGCTATACGAAGTTAT
attB2	mutant version of attB	—	491..516	Rev
DNA Sequence GCCACTTTGTACAAGAAAGCTGGGT