pET22b_PstS_3

78200 Escherichia coli Bacterial Expression

Plasmid Information

Purpose
Expression of double-cysteine variant of mature phosphate binding protein, PstS, which also has a mutation to weaken phosphate binding, as scaffold for phosphate biosensor
Depositor
Martin Webb
Article
Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62
Insert
pstS (A17C,I76G,A197C)
Mutation
A17C,I76G,A197C (please see depositor comment bel…
Promoter
T7
Available Since
July 11, 2016
Availability
Available

Sequence Information

Length
926 bp
Topology
linear
Molecule Type
genomic DNA

Features

No features annotated for this plasmid.