pGEX4T-HEPN

75296 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Expresses Sacsin HEPN domain (4441-4579) in fusion with GST (bacterial)
Depositor
Kalle Gehring
Article
Kozlov et al J Biol Chem. 2011 Jun 10;286(23):20407-1
Insert
Human Sacsin 4441-4579
Tags
GST-Tag
Available Since
July 18, 2016
Availability
Available

Sequence Information

Length
990 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 1
Label Gene Product Position Strand
thrombin site thrombin recognition and cleavage site 8..26 Fwd
DNA Sequence
CTGGTTCCGCGTGGATCC
Protein Sequence
LVPRGS
promoter 1
Label Gene Product Position Strand
AmpR promoter bla 767..872 Fwd
DNA Sequence
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT