ZIKV_NASBA_32B — PlasmidDB

Plasmid Information

Purpose

Portion of Zika Virus genome (KU312312: 7066-7399) containing sensor 32B trigger sequence and NASBA amplification sites

Depositor

James Collins, Alexander Green

Article

Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

Insert

ZIKV_NASBA_32B

Promoter

Available Since

April 5, 2017

Availability

Available

Length

796 bp

Topology

linear

Molecule Type

genomic DNA

promoter 2

Label	Gene	Product	Position	Strand
tet promoter	—	—	522..551	Rev
DNA Sequence TTGACAGCTTATCATCGATAAGCTTTAAT
AmpR promoter	bla	—	663..768	Fwd
DNA Sequence CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT

terminator 1

Label	Gene	Product	Position	Strand
T7 terminator	—	—	301..348	Fwd
DNA Sequence TAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG