pEMI91

74888 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Shuffled 9I27 polyprotein with shuffled codons to allow facile sequencing and unique RE for the 9 cassettes for versatile cloning. See Depositor Comments for more information
Depositor
Piotr Marszalek
Article
Scholl et al Biomacromolecules. 2016 Jun 8.
Insert
Titin I27 x 9 (TTN Human)
Tags
His-tag and Strep-tag II
Promoter
T7 promoter
Available Since
June 13, 2016
Availability
Available

Sequence Information

Length
943 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 2
Label Gene Product Position Strand
6xHis 6xHis affinity tag 43..61 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
thrombin site thrombin recognition and cleavage site 70..88 Fwd
DNA Sequence
CTGGTGCCGCGCGGCAGC
Protein Sequence
LVPRGS