pET28a-FIP3

74753 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Expressed sfGFP-tagged FIP3 in E. coli for protein production
Depositor
Ron Vale
Article
Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554
Insert
Rab11 family interacting protein 3 (RAB11FIP3 Human)
Tags
6x His, Strep II, and superfolder GFP
Promoter
T7
Available Since
June 13, 2016
Availability
Available

Sequence Information

Length
929 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 1
Label Gene Product Position Strand
6xHis 6xHis affinity tag 36..54 Rev
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH