pJM100: no promoter RFP on pCM66T

74739 Synthetic Bacterial Expression

Plasmid Information

Purpose
pCM66T RFP control vector: no promoter driving maxRFP (BBa_E1010)
Depositor
Mary Lidstrom
Insert
RFP (BioBrick part number BBa_E1010)
Promoter
none
Available Since
May 16, 2016
Availability
Available

Sequence Information

Length
1,116 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

protein_bind 1
Label Gene Product Position Strand
CAP binding site — — 1032..1054 Fwd
DNA Sequence
TAATGTGAGTTAGCTCACTCAT
rep_origin 1
Label Gene Product Position Strand
ori — — 157..745 Fwd
DNA Sequence
TTGAGATCCTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA