BRD1A

74666 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Bacterial expression for structure determination; may not be full ORF
Depositor
Nicola Burgess-Brown
Insert
BRD1A (BRD1 Human)
Tags
His-6-TEV
Promoter
T7
Available Since
April 21, 2016
Availability
Available

Sequence Information

Length
777 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 3
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 14..32 Rev
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
TEV site — tobacco etch virus (TEV) protease recog… 478..499 Rev
DNA Sequence
GAGAACCTGTACTTCCAATCC
Protein Sequence
ENLYFQS
6xHis — 6xHis affinity tag 523..541 Rev
DNA Sequence
CACCATCATCATCATCAT
Protein Sequence
HHHHHH
promoter 1
Label Gene Product Position Strand
T7 promoter — — 613..632 Rev
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 1
Label Gene Product Position Strand
lac operator — — 588..613 Fwd
DNA Sequence
GGAATTGTTATCCGCTCACAATTCC