pSG4K5 — PlasmidDB

Plasmid Information

Purpose

For cloning and constitutive expression of sgRNAs in E.coli. New sgRNAs are cloned into SapI sites and multiplex sgRNAs are cloned into BsaI sites. Kanamycin resistant with pSC101 origin of rep.

Depositor

Xiao Wang

Article

Standage-Beier et al ACS Synth Biol. 2015 Nov 20;4(11):1217-2

Insert

sgRNA

Promoter

J23119

Available Since

May 16, 2016

Availability

Available

Sequence Information

Length

907 bp

Topology

linear

Molecule Type

genomic DNA

View on AddGene.org

Features

promoter 1

Label	Gene	Product	Position	Strand
J23119 promoter	—	—	79..114	Fwd
DNA Sequence TTGACAGCTAGCTCAGTCCTAGGTATAATGCTAGC

terminator 1

Label	Gene	Product	Position	Strand
tonB terminator	—	—	312..344	Fwd
DNA Sequence AGTCAAAAGCCTCCGACCGGAGGCTTTTGACT