All_in_one_CRISPR/Cas9_LacZ

74293 Synthetic Bacterial Expression

Plasmid Information

Purpose
"All-in-one" CRISPR/Cas9 (wt) plasmid for cloning of custom gRNA with blue/white screening
Depositor
Lynne Postovit
Article
Findlay et al PLoS One. 2016 Apr 18;11(4):e0153901. do
Tags
HA
Promoter
Lac promoter, SV40, and T7 promoter
Available Since
May 11, 2016
Availability
Available

Sequence Information

Length
988 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
lacZ-alpha lacZ fragment LacZ-alpha fragment of beta-galactosida… 480..759 Fwd
DNA Sequence
ATGACCATGATTACGCCAAGCGCGCAATTAACCCTCACTAAAGGGAACAAAAGCTGGAGGACCTCGAGGGGGGGCCCGGTACCCAATTCGCCCTATAGTGAGTCGTATTACGCGCGCTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGTAG
Protein Sequence
MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEW
promoter 3
Label Gene Product Position Strand
lac promoter — — 406..437 Fwd
DNA Sequence
TTTACACTTTATGCTTCCGGCTCGTATGTTG
T3 promoter — — 505..524 Fwd
DNA Sequence
AATTAACCCTCACTAAAGG
T7 promoter — — 571..590 Rev
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 2
Label Gene Product Position Strand
CAP binding site — — 370..392 Fwd
DNA Sequence
TAATGTGAGTTAGCTCACTCAT
lac operator — — 444..461 Fwd
DNA Sequence
TTGTGAGCGGATAACAA