pTU2S-a (colE1 origin)

74091 Synthetic Bacterial Expression

Plasmid Information

Purpose
Secondary module (BpiI) site in pTU2-a backbone with colE1 (medium-copy) origin of replication
Depositor
Paul Freemont
Article
Moore et al ACS Synth Biol. 2016 May 2.
Insert
Golden Gate destination vector - Level 2, 2 TU's with secondary module and colE1 origin
Promoter
None
Available Since
April 30, 2018
Availability
Available

Sequence Information

Length
838 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

terminator 1
Label Gene Product Position Strand
his operon terminator — — 270..328 Fwd
DNA Sequence
TCCGGCAAAAAAGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAACCGAAAAGA