pET-15b_LC3B

73949 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
For recombinant expression of human LC3B/MAP1LC3B in E. coli
Depositor
Dieter Willbold
Article
Ma et al J Biol Chem. 2013 Dec 27;288(52):37204-1
Insert
LC3B (MAP1LC3B Human)
Tags
His6
Promoter
T7
Available Since
March 25, 2016
Availability
Available

Sequence Information

Length
761 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 3
Label Gene Product Position Strand
6xHis 6xHis affinity tag 32..50 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
thrombin site thrombin recognition and cleavage site 59..77 Fwd
DNA Sequence
CTGGTGCCGCGCGGATCC
Protein Sequence
LVPRGS
LC3B MAP1LC3B human LC3 protein 77..452 Fwd
DNA Sequence
ATGCCGTCGGAGAAGACCTTCAAGCAGCGCCGCACCTTCGAACAAAGAGTAGAAGATGTCCGACTTATTCGAGAGCAGCATCCAACCAAAATCCCGGTGATAATAGAACGATACAAGGGTGAGAAGCAGCTTCCTGTTCTGGATAAAACAAAGTTCCTTGTACCTGACCATGTCAACATGAGTGAGCTCATCAAGATAATTAGAAGGCGCTTACAGCTCAATGCTAATCAGGCCTTCTTCCTGTTGGTGAACGGACACAGCATGGTCAGCGTCTCCACACCAATCTCAGAGGTGTATGAGAGTGAGAAAGATGAAGATGGATTCCTGTACATGGTCTATGCCTCCCAGGAGACGTTCGGGATGAAATTGTCAGTG
Protein Sequence
MPSEKTFKQRRTFEQRVEDVRLIREQHPTKIPVIIERYKGEKQLPVLDKTKFLVPDHVNMSELIKIIRRRLQLNANQAFFLLVNGHSMVSVSTPISEVYESEKDEDGFLYMVYASQETFGMKLSV
terminator 1
Label Gene Product Position Strand
T7 terminator 533..581 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG