pET151-GFP-PP-RPN2 (918-947)

73747 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Expression of GFP-PP-RPN2 (918-947) fusion in E.coli
Depositor
Chris Hill
Article
VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.
Insert
RPN2 (RPN2 Human)
Tags
6x His and GFP
Mutation
Construct possesses only residues 918-947 of RPN2
Promoter
T7
Available Since
April 27, 2017
Availability
Available

Sequence Information

Length
745 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 3
Label Gene Product Position Strand
6xHis 6xHis affinity tag 17..35 Fwd
DNA Sequence
CATCATCACCATCACCAT
Protein Sequence
HHHHHH
V5 tag epitope tag from simian virus 5 35..77 Fwd
DNA Sequence
GGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACG
Protein Sequence
GKPIPNPLLGLDST
TEV site tobacco etch virus (TEV) protease recog… 77..98 Fwd
DNA Sequence
GAAAACCTGTATTTTCAGGGA
Protein Sequence
ENLYFQG