pET151-HIS-TEV-GST-PP-RPN13

73741 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Expression of hRPN13 in E.coli
Depositor
Chris Hill
Article
VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.
Insert
RPN13 (ADRM1 Human)
Tags
6xHIS and GST
Promoter
T7
Available Since
April 27, 2017
Availability
Available

Sequence Information

Length
845 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 5
Label Gene Product Position Strand
6xHis 6xHis affinity tag 34..52 Fwd
DNA Sequence
CATCATCACCATCACCAT
Protein Sequence
HHHHHH
V5 tag epitope tag from simian virus 5 52..94 Fwd
DNA Sequence
GGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACG
Protein Sequence
GKPIPNPLLGLDST
TEV site tobacco etch virus (TEV) protease recog… 94..115 Fwd
DNA Sequence
GAAAACCTGTATTTTCAGGGA
Protein Sequence
ENLYFQG
GST glutathione S-transferase from Schistos… 115..766 Fwd
DNA Sequence
TCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCACTCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATGAGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTGGAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACAGTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTGGTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTGGATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAACTCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCGAAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCATCCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCCAATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTGAAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCATGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCCAAAA
Protein Sequence
SPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK
HRV 3C site recognition and cleavage site for human… 772..796 Fwd
DNA Sequence
CTGGAAGTTCTGTTCCAGGGGCCA
Protein Sequence
LEVLFQGP