pNIA-CEN-FLAG-LINXa4-Bre1

73617 Synthetic Yeast Expression

Plasmid Information

Purpose
Control of the E3 ligase Bre1 with LINXa in yeast.
Depositor
Brian Kuhlman
Article
Yumerefendi et al Nat Chem Biol. 2016 Jun;12(6):399-401. d
Insert
LINXa4-Bre1dNLS
Tags
FLAG
Mutation
Bre1 point mutations K8A, K9A and K11A
Promoter
ADH1
Available Since
April 27, 2016
Availability
Available

Sequence Information

Length
840 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 2
Label Gene Product Position Strand
FLAG FLAG(R) epitope tag, followed by an ent… 189..213 Fwd
DNA Sequence
GATTACAAGGACGACGATGACAAG
Protein Sequence
DYKDDDDK
LOV2 NPH1 from Avena sativa light, oxygen, or voltage domain from p… 264..693 Fwd
DNA Sequence
TTGGCTACTACACTTGAACGTATTGAGAAGAACTTTGTCATTACTGACCCAAGATTGCCAGATAATCCCATTATATTCGCGTCCGATAGTTTCTTGCAGTTGACAGAATATAGCCGTGAAGAAATTTTGGGAAGAAACTGCAGGTTTCTACAAGGTCCTGAAACTGATCGCGCGACAGTGAGAAAAATTAGAGATGCCATAGATAACCAAACAGAGGTCACTGTTCAGCTGATTAATTATACAAAGAGTGGTAAAAAGTTCTGGAACCTCTTTCACTTGCAGCCTATGCGAGATCAGAAGGGAGATGTCCAGTACTTTATTGGGGTTCAGTTGGATGGAACTGAGCATGTCCGAGATGCTGCCGAGAGAGAGGGAGTCATGCTGATTAAGAAAACTGCAGAAAATATTGATGAAGCGGCAAAAGAGCTG
Protein Sequence
LATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGRNCRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNLFHLQPMRDQKGDVQYFIGVQLDGTEHVRDAAEREGVMLIKKTAENIDEAAKEL