pTriEx-mCherry-LINXa3-nano

73616 Synthetic Bacterial Expression

Plasmid Information

Purpose
Light-induced nuclear export switch fusion to nano for microscopy in mammalian cells.
Depositor
Brian Kuhlman
Article
Yumerefendi et al Nat Chem Biol. 2016 Jun;12(6):399-401. d
Insert
LINXa3-nano
Tags
mCherry
Available Since
April 27, 2016
Availability
Available

Sequence Information

Length
888 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 3
Label Gene Product Position Strand
8xHis 8xHis affinity tag 42..66 Rev
DNA Sequence
CACCACCATCACCATCACCATCAC
Protein Sequence
HHHHHHHH
HSV tag HSV (herpes simplex virus) epitope tag 72..105 Rev
DNA Sequence
CAGCCAGAACTCGCCCCGGAAGACCCCGAGGAT
Protein Sequence
QPELAPEDPED
SspB nano SspB from Haemophilus influenzae, with … 147..477 Rev
DNA Sequence
AGCTCCCCGAAACGCCCTAAGCTGCTGCGTGAATATTACGATTGGCTGGTTGATAACAGCTTTACCCCATATCTGGTGGTGGATGCCACATACCTGGGCGTGAACGTGCCCGTGGAGTATGTGAAAGACGGTCAGATCGTGCTGAATCTGTCTGCAAGTGCGACCGGCAACCTGCAACTGACAAATGATTTTATCCAGTTCAACGCCCGCTTTAAGGGCGTGTCTCGTGAACTGTATATCCCGATGGGTGCCGCTCTGGCCATTTACGCTCGCGAGAACGGCGATGGTGTGATGTTCGAACCAGAAGAAATCTATGACGAGCTGAATATT
Protein Sequence
SSPKRPKLLREYYDWLVDNSFTPYLVVDATYLGVNVPVEYVKDGQIVLNLSASATGNLQLTNDFIQFNARFKGVSRELYIPMGAALAIYARENGDGVMFEPEEIYDELNI