pETM6-5F5-mCherry

73422 Synthetic Bacterial Expression

Plasmid Information

Purpose
Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 5F5.
Depositor
Mattheos Koffas
Article
Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
Insert
Promoter 5F5 (orthogonal T7-lac variant)
Promoter
P5F5 (orthogonal T7-lac variant)
Available Since
March 14, 2016
Availability
Available

Sequence Information

Length
751 bp
Topology
linear
Molecule Type
genomic DNA

Features

protein_bind 1
Label Gene Product Position Strand
lac operator 76..101 Fwd
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC