pETM6-At4CL-PhCHS-CmCHI (C5 mutant, pFlavo-opt)

73404 Arabidopsis thaliana Bacterial Expression

Plasmid Information

Purpose
Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo
Depositor
Mattheos Koffas
Article
Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.
Promoter
T7
Available Since
March 1, 2016
Availability
Available

Sequence Information

Length
792 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

promoter 1
Label Gene Product Position Strand
T7 promoter — — 52..71 Fwd
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 1
Label Gene Product Position Strand
lac operator — — 71..96 Fwd
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC