pCfB3052(gRNA X-4, XI-3, XII-5)

73294 Saccharomyces cerevisiae Yeast Expression

Plasmid Information

Purpose
EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-4, XI-3, and XII-5
Depositor
Irina Borodina
Article
Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi
Insert
guiding RNA
Available Since
Nov. 23, 2016
Availability
Available

Sequence Information

Length
1,024 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

promoter 1
Label Gene Product Position Strand
TEF promoter — — 146..490 Fwd
DNA Sequence
GACATGGAGGCCCAGAATACCCTCCTTGACAGTCTTGACGTGCGCAGCTCAGGGGCATGATGTGACTGTCGCCCGTACATTTAGCCCATACATCCCCATGTATAATCATTTGCATCCATACATTTTGATGGCCGCACGGCGCGAAGCAAAAATTACGGCTCCTCGCTGCAGACCTGCGAGCAGGGAAACGCTCCCCTCACAGACGCGTTGAATTGTCCCCACGCCGCGCCCCTGTAGAGAAATATAAAAGGTTAGGATTTGCCACTGAGGTTCTTCTTTCATATACTTCCTTTTAAAATCTTGCTAGGATACAGTTCTCACATCACATCCGAACATAAACAACC