pET21-10XHis-GST-HRV-dL5

73214 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Bacterial expression vector for dL5(E52D) protein with N-terminal His10, GST and HRV3C cleavage site (MBIC5, dL5**, FAP)
Depositor
Marcel Bruchez
Article
Wang et al Bioconjug Chem. 2015 Jan 21;26(1):137-44
Insert
EGFR (EGFR Human, Schistosoma japonicum)
Tags
10XHis-GST-HRV
Promoter
T7
Available Since
March 9, 2016
Availability
Available

Sequence Information

Length
1,025 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 3
Label Gene Product Position Strand
10xHis — 10xHis affinity tag 157..187 Fwd
DNA Sequence
CACCATCATCACCACCATCATCACCACCAT
Protein Sequence
HHHHHHHHHH
GST — glutathione S-transferase from Schistos… 190..844 Fwd
DNA Sequence
ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCACTCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATGAGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTGGAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACAGTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTGGTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTGGATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAACTCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCGAAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCATCCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCCAATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTGAAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCATGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGTGACCATCCTCCAAAA
Protein Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK
HRV 3C site — recognition and cleavage site for human… 865..889 Fwd
DNA Sequence
CTGGAAGTTCTGTTCCAGGGTCCG
Protein Sequence
LEVLFQGP
promoter 1
Label Gene Product Position Strand
T7 promoter — — 64..83 Fwd
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 1
Label Gene Product Position Strand
lac operator — — 83..108 Fwd
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC