pET15b His6-FKBPF36V

73180 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Expressing FKBPF36V protein in washing out shield-1 ligand for FKBPdd
Depositor
Thomas Wandless
Article
Egeler et al J Biol Chem. 2011 Sep 9;286(36):31328-36
Insert
FKBP(F36V) (FKBP1A Human)
Tags
His 6x
Mutation
F36V
Available Since
Feb. 22, 2016
Availability
Available

Sequence Information

Length
726 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 4
Label Gene Product Position Strand
6xHis 6xHis affinity tag 27..45 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
thrombin site thrombin recognition and cleavage site 54..72 Fwd
DNA Sequence
CTGGTGCCGCGCGGCAGC
Protein Sequence
LVPRGS
TEV site tobacco etch virus (TEV) protease recog… 78..99 Fwd
DNA Sequence
GAAAACCTGTATTTTCAGGGA
Protein Sequence
ENLYFQG
FKBP-V F36V mutant of FK506-binding protein FK… 111..438 Fwd
DNA Sequence
ATGGGAGTGCAGGTGGAAACCATCTCCCCAGGAGACGGGCGCACCTTCCCCAAGCGCGGCCAGACCTGCGTGGTGCACTACACCGGGATGCTTGAAGATGGAAAGAAAGTTGATTCCTCCCGGGACAGAAACAAGCCCTTTAAGTTTATGCTAGGCAAGCAGGAGGTGATCCGAGGCTGGGAAGAAGGGGTTGCCCAGATGAGTGTGGGTCAGAGAGCCAAACTGACTATATCTCCAGATTATGCCTATGGTGCCACTGGGCACCCAGGCATCATCCCACCACATGCCACTCTCGTCTTCGATGTGGAGCTTCTAAAACTGGAATGA
Protein Sequence
MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKPFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELLKLE
terminator 1
Label Gene Product Position Strand
T7 terminator 502..550 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG