pDZ645 pKAN 1x-mCherry-12xPP7 V4

Plasmid Information

Purpose

Floxed Kanamycin (G418) selection maker; 3' tagging vector used to add 1x-mCherry followed by 12 repetitive PP7 version 4 RNA stem-loops to gene of interest

Depositor

Daniel Zenklusen

Article

Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

Insert

1x-mCherry

Available Since

Aug. 8, 2016

Availability

Available

Sequence Information

Length

979 bp

Topology

linear

Molecule Type

genomic DNA

View on AddGene.org

Features

promoter 1

Label	Gene	Product	Position	Strand
TEF promoter	—	—	38..382	Rev
DNA Sequence GACATGGAGGCCCAGAATACCCTCCTTGACAGTCTTGACGTGCGCAGCTCAGGGGCATGATGTGACTGTCGCCCGTACATTTAGCCCATACATCCCCATGTATAATCATTTGCATCCATACATTTTGATGGCCGCACGGCGCGAAGCAAAAATTACGGCTCCTCGCTGCAGACCTGCGAGCAGGGAAACGCTCCCCTCACAGACGCGTTGAATTGTCCCCACGCCGCGCCCCTGTAGAGAAATATAAAAGGTTAGGATTTGCCACTGAGGTTCTTCTTTCATATACTTCCTTTTAAAATCTTGCTAGGATACAGTTCTCACATCACATCCGAACATAAACAACC

protein_bind 1

Label	Gene	Product	Position	Strand
loxP	—	—	664..698	Rev
DNA Sequence ATAACTTCGTATAATGTATGCTATACGAAGTTAT

terminator 1

Label	Gene	Product	Position	Strand
ADH1 terminator	S. cerevisiae ADH1	—	451..639	Rev
DNA Sequence GCGAATTTCTTATGATTTATGATTTTTATTATTAAATAAGTTATAAAAAAAATAAGTGTATACAAATTTTAAAGTGACTCTTAGGTTTTAAAACGAAAATTCTTATTCTTGAGTAACTCTTTCCTGTAGGTCAGGTTGCTTTCTCAGGTATAGTATGAGGTCGCTCTTATTGACCACACCTCTACCGG