D282N Cus2p

73060 Saccharomyces cerevisiae Bacterial Expression

Plasmid Information

Purpose
S. cerevisiae Cus2p gene with D282N Mutation
Depositor
Aaron Hoskins
Article
Rodgers et al RNA. 2015 Dec 2.
Insert
CUS2p (CUS2 Budding Yeast)
Tags
6x His tag fused to TEV cleavage site
Mutation
Asp 282 to Asn
Promoter
T7
Availability
Available

Sequence Information

Length
857 bp
Topology
linear
Molecule Type
genomic DNA

Features

promoter 1
Label Gene Product Position Strand
tet promoter 139..168 Fwd
DNA Sequence
TTGACAGCTTATCATCGATAAGCTTTAAT
terminator 1
Label Gene Product Position Strand
T7 terminator 342..391 Rev
DNA Sequence
CTAGCATAACCCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG