L284F Cus2p

73059 Saccharomyces cerevisiae Bacterial Expression

Plasmid Information

Purpose
S. cerevisiae Cus2p gene with L284F Mutation
Depositor
Aaron Hoskins
Article
Rodgers et al RNA. 2015 Dec 2.
Insert
CUS2p (CUS2 Budding Yeast)
Tags
6x His tag fused to TEV cleavage site
Mutation
Leucine 284 to Phenylalanine
Promoter
T7
Availability
Available

Sequence Information

Length
926 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

promoter 1
Label Gene Product Position Strand
tet promoter — — 139..168 Fwd
DNA Sequence
TTGACAGCTTATCATCGATAAGCTTTAAT
terminator 1
Label Gene Product Position Strand
T7 terminator — — 342..391 Rev
DNA Sequence
CTAGCATAACCCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG