wt Cus2p

73046 Saccharomyces cerevisiae Bacterial Expression

Plasmid Information

Purpose
Wild-type S. cerevisiae Cus2p gene for recombinant expression and crystallization
Depositor
Aaron Hoskins
Article
Rodgers et al RNA. 2015 Dec 2.
Insert
CUS2p (CUS2 Budding Yeast)
Tags
6x His tag fused to TEV cleavage site
Promoter
T7
Available Since
May 14, 2019
Availability
Available

Sequence Information

Length
999 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 485..503 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
TEV site — tobacco etch virus (TEV) protease recog… 515..536 Fwd
DNA Sequence
GAAAACTTGTATTTCCAGGGC
Protein Sequence
ENLYFQG
promoter 2
Label Gene Product Position Strand
lacI promoter lacI — 0..78 Rev
DNA Sequence
GACACCATTGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAAT
T7 promoter — — 386..405 Fwd
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 1
Label Gene Product Position Strand
lac operator — — 405..430 Fwd
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC