pET28-Mff(1-61)-PP-GST

73042 Mus musculus Bacterial Expression

Plasmid Information

Purpose
Bacterial expression of mouse Mff residues 1-61 with C-terminal GST-tag and PreScission protease cleavage site
Depositor
David Chan
Article
Liu et al Mol Biol Cell. 2015 Dec 1;26(24):4466-77
Insert
Mff(1-61) (Mff Mouse)
Tags
GST-tag and PreScission protease site
Mutation
Truncation containing amino acids 1-61
Promoter
T7
Available Since
Feb. 17, 2016
Availability
Available

Sequence Information

Length
894 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
HRV 3C site — recognition and cleavage site for human… 667..691 Fwd
DNA Sequence
CTGGAAGTTCTGTTCCAGGGGCCC
Protein Sequence
LEVLFQGP
promoter 2
Label Gene Product Position Strand
lacI promoter lacI — 1..79 Rev
DNA Sequence
GACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAAT
T7 promoter — — 387..406 Fwd
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 1
Label Gene Product Position Strand
lac operator — — 406..431 Fwd
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC