pEN_TT 3XFLAG-S37A-GATA6-3XAU1_RNAi resistant

72924 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Gateway entry vector for an inducible N-terminally 3XFLAG-tagged and C-terminally AU1-tagged, RNAi resistant human S37A-GATA6
Depositor
Kevin Janes
Article
Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:
Insert
GATA Binding Protein 6 (GATA6 Human)
Tags
3XAU1 and 3XFLAG
Mutation
Serine#37 changed to Alanine;Silent mutations mad…
Promoter
TRE tight
Available Since
July 12, 2016
Availability
Available

Sequence Information

Length
563 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

protein_bind 1
Label Gene Product Position Strand
attL2 mutant version of attL — 8..108 Fwd
DNA Sequence
CAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATAAGCAATGCTTTCTTATAATGCCAACTTTGTACAAGAAAGCTGGGT