pEN_TT 3XFLAG-S37A-GATA6-3XAU1

72613 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Gateway entry vector for an inducible N-terminally 3XFLAG-tagged and C-terminally AU1-tagged S37A mutant human GATA6
Depositor
Kevin Janes
Article
Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:
Insert
GATA binding protein 6 (GATA6 Human)
Tags
3XAU1 and 3XFLAG
Mutation
Changed serine37 to alanine
Promoter
TRE tight promoter
Available Since
July 12, 2016
Availability
Available

Sequence Information

Length
3,506 bp
Topology
circular
Molecule Type
other DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
3xFLAG — three tandem FLAG(R) epitope tags, foll… 521..593 Fwd
DNA Sequence
GACTACAAGGATGACGATGACAAGGACTACAAGGATGACGATGACAAGGACTACAAGGATGACGATGACAAG
Protein Sequence
DYKDDDDKDYKDDDDKDYKDDDDK
10xHis — 10xHis affinity tag 1562..1592 Fwd
DNA Sequence
CACCACCACCACCACCACCACCACCACCAT
Protein Sequence
HHHHHHHHHH
promoter 1
Label Gene Product Position Strand
tight TRE promoter — — 197..512 Fwd
DNA Sequence
GAGTTTACTCCCTATCAGTGATAGAGAACGTATGTCGAGTTTACTCCCTATCAGTGATAGAGAACGATGTCGAGTTTACTCCCTATCAGTGATAGAGAACGTATGTCGAGTTTACTCCCTATCAGTGATAGAGAACGTATGTCGAGTTTACTCCCTATCAGTGATAGAGAACGTATGTCGAGTTTATCCCTATCAGTGATAGAGAACGTATGTCGAGTTTACTCCCTATCAGTGATAGAGAACGTATGTCGAGGTAGGCGTGTACGGTGGGAGGCCTATATAAGCAGAGCTCGTTTAGTGAACCGTCAGATCGCC
protein_bind 9
Label Gene Product Position Strand
attL1 mutant version of attL — 66..166 Fwd
DNA Sequence
CAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATAAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGGCT
tet operator tetO — 205..224 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 241..260 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 276..295 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 312..331 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 348..367 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 383..402 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 419..438 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
attL2 mutant version of attL — 2470..2570 Rev
DNA Sequence
CAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATAAGCAATGCTTTCTTATAATGCCAACTTTGTACAAGAAAGCTGGGT